In contrast, the use of GFP is mainly only on non – mammalian vectors. Therefore, we can use it on mammalian vectors. Hence, EFGP has the higher signal strength and higher sensitivity. It is a mutant of the wild-type with improved characteristics. In contrast, EFGP is an enhanced form of GFP protein. The protein was initially in isolation from the jellyfish Aequorea victoria. GFP is the wild-type protein, which is a green fluorescent protein.

GFP and EGFP are reporter proteins in molecular cloning and gene expression studies. The below infographic presents the difference between GFP and EGFP in tabular form. Non-mammalian systems use GFP while mammalian systems use EGFP. Another difference between GFP and EGFP is the systems in which we can use these. For example, EGFP produces stronger fluorescent light and is more sensitive than the GFP. Furthermore, EGFP has more beneficial characteristics than GFP. However, the key difference between GFP and EGFP is that the GFP is a wild-type while EGFP is an engineered version of GFP. Here, GFP and EGFP are two types of green florescent proteins that work as reporter proteins. It signals the successful transformation of the recombinant vector to the host. The reporter gene is a gene that attaches with the gene of interest in recombinant DNA technology. What is the Difference Between GFP and EGFP?

Moreover, it is easy to further mutate these two types to synthesize improved forms.

Also, it is possible to synthesize both using recombinant DNA technology.

Therefore, both function as reporter proteins in gene expression studies.

GFP and EGFP are two proteins which have the ability to emit green colour.

What are the Similarities Between GFP and EGFP? In simple words, fluorescence emission signals the successful transformation of the vector that carries the gene of interest into the host. If the fluorescence results after the transformation process, it confirms the success of the transformation process. Therefore, when inserted into the transformants, it expresses and produces fluorescence. The wild-type gene of GFP is capable of producing a protein which gives fluorescence at room temperature or under UV light. Consequently, scientists used this mutant form of the wild-type gene as a reporter gene in their gene expression studies. However, in natural phenomena, the jellyfish was able to produce green colour fluorescence in response to certain stimuli.Įarlier, this concept surprised the scientists, and they decided to use it on their recombinant DNA technologies. Furthermore, this wild-type protein was originally in isolation from Aequorea Victoria a type of jellyfish. GFP is a wild-type protein that contains 238 amino acid residues and several selectable regions of amino acid sequences that distinguish it from the other fluorescent proteins. Side by Side Comparison – GFP vs EGFP in Tabular Form Thus, the Green Fluorescent Protein (GFP) and Enhanced Green Fluorescent Protein (EGFP) are two reporter proteins used in molecular cloning. These reporter genes act as selectable markers to select the correct transformants.

Out of those techniques, one is reporter gene. To assess this, molecular biologists adopt several techniques. Hence, during the transformation process, it should be identified and confirmed whether the gene of interest has been transformed or not to the host. In recombinant DNA technology, it is necessary to transform recombinant vector successfully to host organism. Molecular cloning is an advanced technique that scientists use immensely in expressing proteins via recombinant technology.

The key difference between GFP and EGFP is that the GFP is a wild-type protein incorporated in the molecular cloning of non-mammalian cells while the EGFP is an improved or engineered type of GFP that can be used on mammalian cells.

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